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atpI [2019-08-12 10:03:54]
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atpI [2019-08-12 10:03:54]

ATP synthase (subunit i)
Locus
BSU_36880
Isoelectric point
10.16
Molecular weight
14.35 kDa
Protein length
127 aa Sequence Blast
Gene length
384 bp Sequence Blast
Function
ATP synthesis
Product
ATP synthase (subunit i))
Essential
no
Synonyms

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,787,620 3,788,003

The protein

Catalyzed reaction/ biological activity

  • ATP synthesis see a video
  • Protein family

  • bacterial atpI family (single member, according to UniProt)
  • Effectors of protein activity

  • ATPase activity is inhibited upon binding of Mg-ADP to AtpD PubMed
  • Localization

  • cell membrane (according to Swiss-Prot)
  • Expression and Regulation

    Operons

    Description

    Regulatory mechanism

  • stringent response: negative regulation, in stringent response
  • Regulation

  • RelA dependent downregulation (Class I) during stringent response PubMed
  • the mRNA is processed between atpI and atpB by RNase Y, this requires the YmcA-YlbF-YaaT complex PubMed
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    Biological materials

    Mutant

  • BKE36880 (atpI::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAATTATCTGTCTCCTG, downstream forward: _UP4_TCAATGGAAGAGAGGTGAAA
  • BKK36880 (atpI::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATCAATTATCTGTCTCCTG, downstream forward: _UP4_TCAATGGAAGAGAGGTGAAA
  • References

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